SCOPE vs STOP
Fit LME models for Prolactin, HPL and GH2.
Fit GEE models for Insulin, HOMA-IR and Glucose.
- Outcomes: Prolactin, hPL, GH2, Insulin, HOMA-IR, Glucose (all outcomes log-transformed)
- Independent variables: Study, GA at sampling
- Model fitted: LME or GEE model
log(hormones) ~ Study + GA at sampling + Study:GA at sampling
Random intercepts for Study ID and Plate
Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
PRL (ng/ml)
|
|
Chisq
|
Df
|
Pr(>Chisq)
|
|
|
(Intercept)
|
21.782244
|
1
|
<0.0001
|
***
|
|
Study
|
1.759986
|
1
|
0.2
|
ns
|
|
GA_samp
|
37.833421
|
1
|
<0.0001
|
***
|
|
Study:GA_samp
|
1.825160
|
1
|
0.2
|
ns
|
hPL (μg/ml)
|
|
Chisq
|
Df
|
Pr(>Chisq)
|
|
|
(Intercept)
|
107.37637
|
1
|
<0.0001
|
***
|
|
Study
|
63.37312
|
1
|
<0.0001
|
***
|
|
GA_samp
|
115.88145
|
1
|
<0.0001
|
***
|
|
Study:GA_samp
|
71.34390
|
1
|
<0.0001
|
***
|
GH2 (ng/ml)
|
|
Chisq
|
Df
|
Pr(>Chisq)
|
|
|
(Intercept)
|
9.974225
|
1
|
0.002
|
**
|
|
Study
|
26.572090
|
1
|
<0.0001
|
***
|
|
GA_samp
|
57.571753
|
1
|
<0.0001
|
***
|
|
Study:GA_samp
|
26.560416
|
1
|
<0.0001
|
***
|
Insulin (uU/L)
|
|
model term
|
df1
|
df2
|
F.ratio
|
Chisq
|
p.value
|
|
|
1
|
Study
|
1
|
Inf
|
3.706
|
3.706
|
0.05
|
ns
|
|
3
|
GA_samp
|
1
|
Inf
|
0.183
|
0.183
|
0.7
|
ns
|
|
2
|
Study:GA_samp
|
1
|
Inf
|
2.665
|
2.665
|
0.1
|
ns
|
HOMA-IR
|
|
model term
|
df1
|
df2
|
F.ratio
|
Chisq
|
p.value
|
|
|
1
|
Study
|
1
|
Inf
|
4.810
|
4.810
|
0.03
|
*
|
|
3
|
GA_samp
|
1
|
Inf
|
0.432
|
0.432
|
0.5
|
ns
|
|
2
|
Study:GA_samp
|
1
|
Inf
|
2.900
|
2.900
|
0.09
|
ns
|
Glucose (mmol/L)
|
|
model term
|
df1
|
df2
|
F.ratio
|
Chisq
|
p.value
|
|
|
1
|
Study
|
1
|
Inf
|
0.138
|
0.138
|
0.7
|
ns
|
|
3
|
GA_samp
|
1
|
Inf
|
0.001
|
0.001
|
1.0
|
ns
|
|
2
|
Study:GA_samp
|
1
|
Inf
|
0.190
|
0.190
|
0.7
|
ns
|
Model summary
Ratio of Geometric means (95% CI)
PRL (ng/ml)
|
Var
|
Effect
|
P
|
|
(Intercept)
|
2.14 (1.24, 3.03)
|
<0.0001
|
|
StudySTOP
|
0.63 (-0.30, 1.56)
|
0.18
|
|
GA_samp
|
0.18 (0.12, 0.24)
|
<0.0001
|
|
StudySTOP:GA_samp
|
-0.04 (-0.10, 0.02)
|
0.18
|
hPL (μg/ml)
|
Var
|
Effect
|
P
|
|
(Intercept)
|
2.53 (2.04, 3.01)
|
<0.0001
|
|
StudySTOP
|
-2.02 (-2.52, -1.52)
|
<0.0001
|
|
GA_samp
|
0.17 (0.14, 0.20)
|
<0.0001
|
|
StudySTOP:GA_samp
|
0.14 (0.11, 0.18)
|
<0.0001
|
GH2 (ng/ml)
|
Var
|
Effect
|
P
|
|
(Intercept)
|
-1.04 (-1.70, -0.38)
|
0.002
|
|
StudySTOP
|
-1.77 (-2.45, -1.08)
|
<0.0001
|
|
GA_samp
|
0.16 (0.12, 0.20)
|
<0.0001
|
|
StudySTOP:GA_samp
|
0.12 (0.07, 0.16)
|
<0.0001
|
Insulin (uU/L)
|
Var
|
Effect
|
P
|
|
(Intercept)
|
3.45 (2.16, 4.73)
|
<0.0001
|
|
StudySTOP
|
-1.31 (-2.64, 0.02)
|
0.05
|
|
GA_samp
|
-0.05 (-0.13, 0.04)
|
0.27
|
|
StudySTOP:GA_samp
|
0.07 (-0.01, 0.16)
|
0.10
|
HOMA-IR
|
Var
|
Effect
|
P
|
|
(Intercept)
|
2.16 (0.83, 3.50)
|
0.001
|
|
StudySTOP
|
-1.55 (-2.94, -0.16)
|
0.03
|
|
GA_samp
|
-0.06 (-0.14, 0.03)
|
0.21
|
|
StudySTOP:GA_samp
|
0.08 (-0.01, 0.17)
|
0.09
|
Glucose (mmol/L)
|
Var
|
Effect
|
P
|
|
(Intercept)
|
1.66 (1.30, 2.02)
|
<0.0001
|
|
StudySTOP
|
-0.07 (-0.43, 0.29)
|
0.71
|
|
GA_samp
|
0.00 (-0.02, 0.03)
|
0.84
|
|
StudySTOP:GA_samp
|
-0.01 (-0.03, 0.02)
|
0.66
|
Diagnostics
Model diagnositcs.
Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/L)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
PRL (ng/ml)
|
Study
|
GA_samp
|
response
|
SE
|
df
|
lower.CL
|
upper.CL
|
|
SCOPE
|
10
|
51.11441
|
8.906805
|
1114.07386
|
36.31267
|
71.94962
|
|
STOP
|
10
|
62.65847
|
4.432004
|
69.44323
|
54.41322
|
72.15314
|
|
SCOPE
|
16
|
150.41614
|
10.759690
|
72.33237
|
130.42768
|
173.46791
|
|
STOP
|
16
|
142.93995
|
12.259227
|
148.33938
|
120.65629
|
169.33912
|
hPL (μg/ml)
|
Study
|
GA_samp
|
response
|
SE
|
df
|
lower.CL
|
upper.CL
|
|
SCOPE
|
10
|
67.92125
|
6.0306146
|
1668.81645
|
57.06556
|
80.84205
|
|
STOP
|
10
|
37.36817
|
0.9095297
|
93.09186
|
35.60500
|
39.21865
|
|
SCOPE
|
16
|
187.35085
|
4.6396951
|
97.63587
|
178.36571
|
196.78863
|
|
STOP
|
16
|
242.41586
|
8.6131317
|
388.30674
|
226.05960
|
259.95557
|
GH2 (ng/ml)
|
Study
|
GA_samp
|
response
|
SE
|
df
|
lower.CL
|
upper.CL
|
|
SCOPE
|
10
|
1.7651770
|
0.2079050
|
779.4183
|
1.4008017
|
2.224333
|
|
STOP
|
10
|
0.9757305
|
0.0225094
|
193.2049
|
0.9323297
|
1.021152
|
|
SCOPE
|
16
|
4.6213763
|
0.1093036
|
180.9841
|
4.4106582
|
4.842161
|
|
STOP
|
16
|
5.1643805
|
0.2209490
|
1235.9833
|
4.7485975
|
5.616569
|
Insulin (uU/L)
|
Study
|
GA_samp
|
response
|
SE
|
df
|
asymp.LCL
|
asymp.UCL
|
|
SCOPE
|
10
|
19.77659
|
4.6279509
|
Inf
|
12.50145
|
31.28545
|
|
STOP
|
10
|
11.15166
|
0.3842101
|
Inf
|
10.42348
|
11.93070
|
|
SCOPE
|
16
|
14.96218
|
0.6124863
|
Inf
|
13.80862
|
16.21210
|
|
STOP
|
16
|
13.12747
|
0.9877112
|
Inf
|
11.32756
|
15.21337
|
HOMA-IR
|
Study
|
GA_samp
|
response
|
SE
|
df
|
asymp.LCL
|
asymp.UCL
|
|
SCOPE
|
10
|
5.004074
|
1.2160703
|
Inf
|
3.107908
|
8.057111
|
|
STOP
|
10
|
2.360224
|
0.0858766
|
Inf
|
2.197770
|
2.534686
|
|
SCOPE
|
16
|
3.587401
|
0.1563751
|
Inf
|
3.293639
|
3.907364
|
|
STOP
|
16
|
2.735166
|
0.2179779
|
Inf
|
2.339631
|
3.197568
|
Glucose (mmol/L)
|
Study
|
GA_samp
|
response
|
SE
|
df
|
asymp.LCL
|
asymp.UCL
|
|
SCOPE
|
10
|
5.395297
|
0.3344260
|
Inf
|
4.778085
|
6.092238
|
|
STOP
|
10
|
4.773670
|
0.0366162
|
Inf
|
4.702440
|
4.845979
|
|
SCOPE
|
16
|
5.475695
|
0.0719187
|
Inf
|
5.336536
|
5.618483
|
|
STOP
|
16
|
4.691147
|
0.0561450
|
Inf
|
4.582385
|
4.802490
|
Comparisons
|
Hormone
|
Contrast
|
GA_samp
|
Ratio of Geometric means (95% CI)
|
P-value
|
|
PRL (ng/ml)
|
STOP / SCOPE
|
10
|
1.23 (0.89, 1.69)
|
0.2
|
|
PRL (ng/ml)
|
STOP / SCOPE
|
16
|
0.95 (0.84, 1.07)
|
0.4
|
|
hPL (μg/ml)
|
STOP / SCOPE
|
10
|
0.55 (0.46, 0.65)
|
<0.0001
|
|
hPL (μg/ml)
|
STOP / SCOPE
|
16
|
1.29 (1.21, 1.38)
|
<0.0001
|
|
GH2 (ng/ml)
|
STOP / SCOPE
|
10
|
0.55 (0.44, 0.70)
|
<0.0001
|
|
GH2 (ng/ml)
|
STOP / SCOPE
|
16
|
1.12 (1.02, 1.22)
|
0.01
|
|
Insulin (uU/L)
|
STOP / SCOPE
|
10
|
0.56 (0.35, 0.90)
|
0.02
|
|
Insulin (uU/L)
|
STOP / SCOPE
|
16
|
0.88 (0.74, 1.04)
|
0.1
|
|
HOMA-IR
|
STOP / SCOPE
|
10
|
0.47 (0.29, 0.76)
|
0.002
|
|
HOMA-IR
|
STOP / SCOPE
|
16
|
0.76 (0.64, 0.91)
|
0.003
|
|
Glucose (mmol/L)
|
STOP / SCOPE
|
10
|
0.88 (0.78, 1.00)
|
0.05
|
|
Glucose (mmol/L)
|
STOP / SCOPE
|
16
|
0.86 (0.83, 0.89)
|
<0.0001
|





